Positional scanning

Positional Scan

Positional scanning is an important tool for peptide sequence optimization. This identifies an amino acid of interest at a single position and substitutes it with all other natural amino acids one at a time. Increases in activity will identify the preferred amino acid residues at this positions.


Special Instructions

Enter your target sequence below as well as the position(s) of the amino acid to be substituted, counting from the N-Terminus. A total of 20 sequences will be generated for each positional replacement.

Peptide Sequence (25 amino acids max) 3 letter converter
Position of residue(s) to be replaced (from Nterm)
Please separate numbers out using semi-colons (;)
The calculation results
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