Mass spectrometry is an analytical technique that ionizes a sample into a gaseous ion mixture and separates it according to the mass-to-charge ratio (m/z).
The simplest use of mass spectrometry is molecular weight determination (click-in).
According to the characteristics of debris ions can also be used for qualitative analysis of analytes.
In addition, quantitative analysis of analytes can be performed according to the peak intensity of mass spectrometry.
The mass spectrometer consists of the following parts
Data and power supply system
┏━━━━┳━━━━━╋━━━━━━┓
Injection ion source mass analyzer Detection receiver
┗━━━━━╋━━━━━━┛
Ion source: a device that ionizes the atoms or molecules of the sample to be analyzed into charged particles (ions) and accelerates the ions to enter the analyzer. According to the different ionization modes, common methods for analysis of biological macromolecules include:
1,ESI(Electrospray Ionization):Electrospray ionization is the softest ionization method
2,MALDI(Matrix Assisted Laser Desorption):Matrix assisted laser desorption ionization
According to the mass analyzer, the following types are common:
Quadrupole mass spectrometer (Q).
Ions TRAP mass spectrometer.
Time of flight mass spectrometer (TOF).
Fourier transform-ion cyclotron resonance mass spectrometer (FT-ICRMS).
- Molecular weight analysis
- Protein mass spectrometry
- Technical service of quality combination
Molecular weight is one of the key characteristic parameters of proteins and the most basic physical and chemical property parameter of organic compounds.
Whether the molecular weight is correct or not often represents whether the structures of the measured organic compounds and biological macromolecules are correct or not. Molecular weight is also the primary parameter in the identification of polypeptides, proteins, etc. With the advancement of genome and proteome research, the scientific research work of protein molecular weight determination has received more and more attention. The following method accurately determines the molecular weight of protein by ESI-TOF. The instrument used, Mass Spectrometer Triplet of 5600 Manufacturer AB Sciex Instruments, is also combined with other molecular weight mass spectrometry methods, and the results are further confirmed.
Mass spectrometry (MS) is a technique to separate compound forming ions and fragment ions according to different mass-to-charge ratios (M/S) for component and structure analysis. The results will be shown by Mass spectrum. According to the information provided by the mass spectrogram, we will carry out structural analysis of biological macromolecules, qualitative and quantitative analysis of organic and inorganic substances.
Bio-mass spectrometry (bio-ms) is a mass spectrometry protein sequencing technology for analyzing biological macromolecules. As is known to all, biological macromolecules are mostly distinguished from inorganic and organic micromolecules by high molecular mass. For the latter, mass spectrometry only requires the determination of relative molecular weights ranging from tens to 2000, while bio-mass spectrometry requires tens or even hundreds of thousands of relative molecular weights, so mass spectrometry suitable for inorganic and organic small molecule analysis cannot be applied to bio-macromolecule analysis. With the improvement and maturity of ESI and matrix assisted laser desorption ionization (MALDI) technologies, mass spectrometry detection and analysis of biological macromolecules can be realized.
Liquid chromatography-mass spectrometry (LC-MS) is a separation and analysis method that uses mass spectrometer as detection means and integrates high separation capability of HPLC with high sensitivity and high selectivity of MS. Especially in recent years, with the maturity of soft ionization technologies such as electrospray and atmospheric pressure chemical ionization, the qualitative and quantitative analysis results of sample protein sequencing are more reliable. At the same time, due to the unique advantages of liquid chromatography-mass spectrometry in the separation and identification of high boiling point, nonvolatile and thermally unstable compounds, Therefore, it has become an indispensable means for analysis of traditional Chinese medicine preparations, pharmacokinetics, food safety testing and clinical medicine research.