Mammalian cell protein expression

Trigoats Co., Ltd. has successfully constructed various complete protein expression technology platforms, including prokaryotic protein expression and purification, yeast protein expression and purification, insect cell protein expression and purification, mammalian cell protein expression and purification.According to customers' needs, we provide one-stop services from gene synthesis, vector construction, gene expression, protein purification, detection, etc.In order to ensure the quality of protein purification, trigoats has established a strict product quality control system and a complete set of perfect customer service process and confidentiality system to ensure to provide customers with high-quality products and mature services. Our protein team has more experience in optimizing gene codon, design and construction of expression vector, selection of fusion object and label, selection of specific protein purification system, optimization of protein production process, etc.Can meet the personalized needs of customers. Mammalian cell protein expression

Mammalian cell protein expression is suitable for large-scale and rapid protein preparation. We provide one-stop protein technology services such as expression vector construction, transient transfection, expression detection, large-scale cell culture expression and protein purification.Among them, it can be divided into transient expression, stable expression and stable expression cell line construction.

We can provide mammalian protein from mg to g.Generally, it can be transfected quickly within 10 days without genetic selection of plasmid DNA.Our transient expression uses suspension cells, which can easily express ml to 100L of protein.About four weeks after the construction of the suitable expression vector, we can deliver the expression product to the customer: cell culture medium collection or recombinant protein.Trigoats can clone the target gene of the customer into a highly efficient expression vector specially designed for tge system if the customer needs it.

1、Cloning target protein coding sequence to mammalian transient expression vector;

2、To prepare plasmids which can be used to transfect mammalian cells;

3、Mammalian cell transfection, evaluation of recombinant protein expression and optimization of conditions;

4、Small scale protein expression and purification (500 ml Culture), protein detection;

5、Large scale protein expression and purification (optional).

The whole experimental process, from transfection, screening and cell separation to evaluation of the production capacity and adaptability of preliminary clones, provides long-term stable and scale adjustable protein production. According to the needs of customers, trigoats Co., Ltd. will carry out gene amplification, cell transfection and storage.We also have comprehensive genetic research ability to ensure the stability of protein production.

1、Clone the coding sequence of the target protein to the mammalian stable expression vector;

2、To prepare plasmids which can be used to transfect mammalian cells;

3、Mammalian cells were transfected and stable transfected cells were screened;

4、Monoclonal screening and expression test of recombinant protein;

5、Small scale protein expression and purification (500 ml Culture), protein detection;

6、Large scale protein expression and purification (optional).

Trigoats Co., Ltd. provides one-stop services such as gene synthesis, cloning, vector design and construction, protein purification and identification, and new drug screening. It can build and screen cell lines that meet your needs, explore faster and better experimental solutions, and help you save a lot of time in the later stage of project planning.

1、Customers can choose the cells they need; they can provide them by themselves, or they can choose from the cell bank of trigoats Co., Ltd., or order them;

2、Clone the coding sequence of the target protein to the appropriate mammalian cell stable expression vector (or the vector designated by the customer);

3、To prepare plasmids which can be used to transfect mammalian cells;

4、Mammalian cells were transfected and stable transfected cells were screened;

5、Monoclonal screening (optional) and evaluation of recombinant protein expression.